Characterization of Human Coronavirus Etiology in Chinese Adults with Acute Upper Respiratory Tract Infection by Real-Time RT-PCR Assays
Identifieur interne : 001D51 ( Main/Exploration ); précédent : 001D50; suivant : 001D52Characterization of Human Coronavirus Etiology in Chinese Adults with Acute Upper Respiratory Tract Infection by Real-Time RT-PCR Assays
Auteurs : Roujian Lu [République populaire de Chine] ; Xiaoyan Yu [République populaire de Chine] ; Wen Wang [République populaire de Chine] ; Xijie Duan [République populaire de Chine] ; Linglin Zhang [République populaire de Chine] ; Weimin Zhou [République populaire de Chine] ; Jin Xu [République populaire de Chine] ; Lingjie Xu [République populaire de Chine] ; Qin Hu [République populaire de Chine] ; Jianxin Lu [République populaire de Chine] ; Li Ruan [République populaire de Chine] ; Zhong Wang [République populaire de Chine] ; Wenjie Tan [République populaire de Chine]Source :
- PLoS ONE [ 1932-6203 ] ; 2012.
Descripteurs français
- KwdFr :
- Amorces ADN, Chine (épidémiologie), Coronavirus (génétique), Coronavirus (isolement et purification), Humains, Infections de l'appareil respiratoire (virologie), Maladie aigüe, Partie nasale du pharynx (virologie), Réaction de polymérisation en chaine en temps réel (), Surveillance de la population, Séquence nucléotidique.
- MESH :
- génétique : Coronavirus.
- isolement et purification : Coronavirus.
- virologie : Infections de l'appareil respiratoire, Partie nasale du pharynx.
- épidémiologie : Chine.
- Amorces ADN, Humains, Maladie aigüe, Réaction de polymérisation en chaine en temps réel, Surveillance de la population, Séquence nucléotidique.
- Wicri :
- geographic : République populaire de Chine.
English descriptors
- KwdEn :
- MESH :
- chemical : DNA Primers.
- geographic , epidemiology : China.
- genetics : Coronavirus.
- isolation & purification : Coronavirus.
- methods : Real-Time Polymerase Chain Reaction.
- virology : Nasopharynx, Respiratory Tract Infections.
- Acute Disease, Base Sequence, Humans, Population Surveillance.
Abstract
In addition to SARS associated coronaviruses, 4 non-SARS related human coronaviruses (HCoVs) are recognized as common respiratory pathogens. The etiology and clinical impact of HCoVs in Chinese adults with acute upper respiratory tract infection (URTI) needs to be characterized systematically by molecular detection with excellent sensitivity.
In this study, we detected 4 non-SARS related HCoV species by real-time RT-PCR in 981 nasopharyngeal swabs collected from March 2009 to February 2011. All specimens were also tested for the presence of other common respiratory viruses and newly identified viruses, human metapneumovirus (hMPV) and human bocavirus (HBoV). 157 of the 981 (16.0%) nasopharyngeal swabs were positive for HCoVs. The species detected were 229E (96 cases, 9.8%), OC43 (42 cases, 4.3%), HKU1 (16 cases, 1.6%) and NL63 (11 cases, 1.1%). HCoV-229E was circulated in 21 of the 24 months of surveillance. The detection rates for both OC43 and NL63 were showed significantly year-to-year variation between 2009/10 and 2010/11, respectively (P<0.001 and P = 0.003), and there was a higher detection frequency of HKU1 in patients aged over 60 years (P = 0.03). 48 of 157(30.57%) HCoV positive patients were co-infected. Undifferentiated human rhinoviruses and influenza (Flu) A were the most common viruses detected (more than 35%) in HCoV co-infections. Respiratory syncytial virus (RSV), human parainfluenza virus (PIV) and HBoV were detected in very low rate (less than 1%) among adult patients with URTI.
All 4 non-SARS-associated HCoVs were more frequently detected by real-time RT-PCR assay in adults with URTI in Beijing and HCoV-229E led to the most prevalent infection. Our study also suggested that all non-SARS-associated HCoVs contribute significantly to URTI in adult patients in China.
Url:
DOI: 10.1371/journal.pone.0038638
PubMed: 22719912
PubMed Central: 3376151
Affiliations:
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id="aff1"><addr-line>National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Institute for Viral Disease Control and Prevention, China CDC, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Key Laboratory of Laboratory Medicine, Ministry of Education, and Institute of Medical Virology, Wenzhou Medical College, Zhejiang, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Laboratory Medicine, Ministry of Education, and Institute of Medical Virology, Wenzhou Medical College, Zhejiang</wicri:regionArea><wicri:noRegion>Zhejiang</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Wen" sort="Wang, Wen" uniqKey="Wang W" first="Wen" last="Wang">Wen Wang</name><affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Institute for Viral Disease Control and Prevention, China CDC, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Duan, Xijie" sort="Duan, Xijie" uniqKey="Duan X" first="Xijie" last="Duan">Xijie Duan</name><affiliation wicri:level="3"><nlm:aff id="aff2"><addr-line>Peking Union Medical College Hospital, Chinese Academy Medical Sciences, Beijing, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Peking Union Medical College Hospital, Chinese Academy Medical Sciences, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhang, Linglin" sort="Zhang, Linglin" uniqKey="Zhang L" first="Linglin" last="Zhang">Linglin Zhang</name><affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Institute for Viral Disease Control and Prevention, China CDC, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhou, Weimin" sort="Zhou, Weimin" uniqKey="Zhou W" first="Weimin" last="Zhou">Weimin Zhou</name><affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Institute for Viral Disease Control and Prevention, China CDC, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Xu, Jin" sort="Xu, Jin" uniqKey="Xu J" first="Jin" last="Xu">Jin Xu</name><affiliation wicri:level="3"><nlm:aff id="aff2"><addr-line>Peking Union Medical College Hospital, Chinese Academy Medical Sciences, Beijing, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Peking Union Medical College Hospital, Chinese Academy Medical Sciences, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Xu, Lingjie" sort="Xu, Lingjie" uniqKey="Xu L" first="Lingjie" last="Xu">Lingjie Xu</name><affiliation wicri:level="3"><nlm:aff id="aff2"><addr-line>Peking Union Medical College Hospital, Chinese Academy Medical Sciences, Beijing, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Peking Union Medical College Hospital, Chinese Academy Medical Sciences, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Hu, Qin" sort="Hu, Qin" uniqKey="Hu Q" first="Qin" last="Hu">Qin Hu</name><affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Institute for Viral Disease Control and Prevention, China CDC, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Key Laboratory of Laboratory Medicine, Ministry of Education, and Institute of Medical Virology, Wenzhou Medical College, Zhejiang, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Laboratory Medicine, Ministry of Education, and Institute of Medical Virology, Wenzhou Medical College, Zhejiang</wicri:regionArea><wicri:noRegion>Zhejiang</wicri:noRegion></affiliation></author><author><name sortKey="Lu, Jianxin" sort="Lu, Jianxin" uniqKey="Lu J" first="Jianxin" last="Lu">Jianxin Lu</name><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Key Laboratory of Laboratory Medicine, Ministry of Education, and Institute of Medical Virology, Wenzhou Medical College, Zhejiang, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Laboratory Medicine, Ministry of Education, and Institute of Medical Virology, Wenzhou Medical College, Zhejiang</wicri:regionArea><wicri:noRegion>Zhejiang</wicri:noRegion></affiliation></author><author><name sortKey="Ruan, Li" sort="Ruan, Li" uniqKey="Ruan L" first="Li" last="Ruan">Li Ruan</name><affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Institute for Viral Disease Control and Prevention, China CDC, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wang, Zhong" sort="Wang, Zhong" uniqKey="Wang Z" first="Zhong" last="Wang">Zhong Wang</name><affiliation wicri:level="3"><nlm:aff id="aff2"><addr-line>Peking Union Medical College Hospital, Chinese Academy Medical Sciences, Beijing, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Peking Union Medical College Hospital, Chinese Academy Medical Sciences, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Tan, Wenjie" sort="Tan, Wenjie" uniqKey="Tan W" first="Wenjie" last="Tan">Wenjie Tan</name><affiliation wicri:level="3"><nlm:aff id="aff1"><addr-line>National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China</addr-line></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Institute for Viral Disease Control and Prevention, China CDC, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acute Disease</term><term>Base Sequence</term><term>China (epidemiology)</term><term>Coronavirus (genetics)</term><term>Coronavirus (isolation & purification)</term><term>DNA Primers</term><term>Humans</term><term>Nasopharynx (virology)</term><term>Population Surveillance</term><term>Real-Time Polymerase Chain Reaction (methods)</term><term>Respiratory Tract Infections (virology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Amorces ADN</term><term>Chine (épidémiologie)</term><term>Coronavirus (génétique)</term><term>Coronavirus (isolement et purification)</term><term>Humains</term><term>Infections de l'appareil respiratoire (virologie)</term><term>Maladie aigüe</term><term>Partie nasale du pharynx (virologie)</term><term>Réaction de polymérisation en chaine en temps réel ()</term><term>Surveillance de la population</term><term>Séquence nucléotidique</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA Primers</term></keywords><keywords scheme="MESH" type="geographic" qualifier="epidemiology" xml:lang="en"><term>China</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Real-Time Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections de l'appareil respiratoire</term><term>Partie nasale du pharynx</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Nasopharynx</term><term>Respiratory Tract Infections</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Chine</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Acute Disease</term><term>Base Sequence</term><term>Humans</term><term>Population Surveillance</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Amorces ADN</term><term>Humains</term><term>Maladie aigüe</term><term>Réaction de polymérisation en chaine en temps réel</term><term>Surveillance de la population</term><term>Séquence nucléotidique</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>République populaire de Chine</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>In addition to SARS associated coronaviruses, 4 non-SARS related human coronaviruses (HCoVs) are recognized as common respiratory pathogens. The etiology and clinical impact of HCoVs in Chinese adults with acute upper respiratory tract infection (URTI) needs to be characterized systematically by molecular detection with excellent sensitivity.</p></sec><sec><title>Methodology/Principal Findings</title><p>In this study, we detected 4 non-SARS related HCoV species by real-time RT-PCR in 981 nasopharyngeal swabs collected from March 2009 to February 2011. All specimens were also tested for the presence of other common respiratory viruses and newly identified viruses, human metapneumovirus (hMPV) and human bocavirus (HBoV). 157 of the 981 (16.0%) nasopharyngeal swabs were positive for HCoVs. The species detected were 229E (96 cases, 9.8%), OC43 (42 cases, 4.3%), HKU1 (16 cases, 1.6%) and NL63 (11 cases, 1.1%). HCoV-229E was circulated in 21 of the 24 months of surveillance. The detection rates for both OC43 and NL63 were showed significantly year-to-year variation between 2009/10 and 2010/11, respectively (P<0.001 and P = 0.003), and there was a higher detection frequency of HKU1 in patients aged over 60 years (P = 0.03). 48 of 157(30.57%) HCoV positive patients were co-infected. Undifferentiated human rhinoviruses and influenza (Flu) A were the most common viruses detected (more than 35%) in HCoV co-infections. Respiratory syncytial virus (RSV), human parainfluenza virus (PIV) and HBoV were detected in very low rate (less than 1%) among adult patients with URTI.</p></sec><sec><title>Conclusions/Significance</title><p>All 4 non-SARS-associated HCoVs were more frequently detected by real-time RT-PCR assay in adults with URTI in Beijing and HCoV-229E led to the most prevalent infection. 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Lu</name></noRegion><name sortKey="Duan, Xijie" sort="Duan, Xijie" uniqKey="Duan X" first="Xijie" last="Duan">Xijie Duan</name><name sortKey="Hu, Qin" sort="Hu, Qin" uniqKey="Hu Q" first="Qin" last="Hu">Qin Hu</name><name sortKey="Hu, Qin" sort="Hu, Qin" uniqKey="Hu Q" first="Qin" last="Hu">Qin Hu</name><name sortKey="Lu, Jianxin" sort="Lu, Jianxin" uniqKey="Lu J" first="Jianxin" last="Lu">Jianxin Lu</name><name sortKey="Ruan, Li" sort="Ruan, Li" uniqKey="Ruan L" first="Li" last="Ruan">Li Ruan</name><name sortKey="Tan, Wenjie" sort="Tan, Wenjie" uniqKey="Tan W" first="Wenjie" last="Tan">Wenjie Tan</name><name sortKey="Wang, Wen" sort="Wang, Wen" uniqKey="Wang W" first="Wen" last="Wang">Wen Wang</name><name sortKey="Wang, Zhong" sort="Wang, Zhong" uniqKey="Wang Z" first="Zhong" last="Wang">Zhong Wang</name><name sortKey="Xu, Jin" sort="Xu, Jin" uniqKey="Xu J" first="Jin" last="Xu">Jin Xu</name><name sortKey="Xu, Lingjie" sort="Xu, Lingjie" uniqKey="Xu L" first="Lingjie" last="Xu">Lingjie Xu</name><name sortKey="Yu, Xiaoyan" sort="Yu, Xiaoyan" uniqKey="Yu X" first="Xiaoyan" last="Yu">Xiaoyan Yu</name><name sortKey="Yu, Xiaoyan" sort="Yu, Xiaoyan" uniqKey="Yu X" first="Xiaoyan" last="Yu">Xiaoyan Yu</name><name sortKey="Zhang, Linglin" sort="Zhang, Linglin" uniqKey="Zhang L" first="Linglin" last="Zhang">Linglin Zhang</name><name sortKey="Zhou, Weimin" sort="Zhou, Weimin" uniqKey="Zhou W" first="Weimin" last="Zhou">Weimin Zhou</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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